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Harnessing malE for the study of antigen/antibody recognitions
Hugues Bedouelle1, Martial Renard, Laurent Belkadi
1Department of Structural Biology and Chemistry, CNRS URA 2185, Institut Pasteur, Paris, France. hbedouel@pasteur.fr
Research in Microbiology
|October 31, 2002
Abstract:
The construction of hybrids between the variable fragment (Fv) of antibodies and protein MalE of Escherichia coli at the genetic level makes possible their preparation in a functional state, independently of any interaction with the antigen. We used such hybrids and a mutagenesis approach to study the recognition between antibody D1.3 and its antigen lysozyme, and its maturation. We subsequently transformed D1.3 into a reagentless fluorescent biosensor by knowledge-based design.