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Positive selection vectors.
Young-Jun Choi1, Tsung-Tsan Wang, Byong H Lee
1Department of Food Science and Agricultural Chemistry, McGill University, Ste.-Anne-de-Bellevue, Quebec, Canada. Choiy@mtl.nrc.ca
Critical Reviews in Biotechnology
|October 31, 2002
Summary
Positive selection vectors simplify screening of DNA fragments in recombinant DNA experiments. They reduce background noise and offer convenient mechanisms like insertional inactivation for direct transformant identification.
Area of Science:
- Molecular Biology
- Biotechnology
Background:
- Positive selection vectors are crucial tools in molecular biology.
- They enable efficient screening of recombinant DNA molecules.
Purpose of the Study:
- To review the mechanisms, classification, properties, and limitations of positive selection vectors.
- To discuss 72 different positive selection vectors.
Main Methods:
- Literature review of existing positive selection vectors.
- Analysis of vector mechanisms, structures, and applications.
Main Results:
- Positive selection vectors reduce background and directly screen transformants.
- Mechanisms include insertional inactivation and functional gene replacement.
- Vectors are classified into five groups based on structure.
- Common mechanisms involve lethal genes and compound sensitivity.
- Diverse host ranges include bacteria, yeast, and mammalian cells.
Conclusions:
- Positive selection vectors are valuable for recombinant DNA experiments despite some limitations.
- Insertional inactivation is generally more convenient than functional gene replacement.