Pro- and macroglycogenolysis in skeletal muscle during maximal treadmill exercise

The purpose was to investigate the degradation of proglycogen and macroglycogen in skeletal muscle during intense exercise. Ten Standardbred trotters performed a maximal treadmill exercise test comprising a warm-up period, an exercise period, starting at 7 m/s with increments of 1 m/s every 60 s until the onset of fatigue (mean +/- s.d. 246 +/- 32 s) and a walking recovery period. Muscle biopsies were taken at rest, immediately after exercise and 15 min postexercise. The exercise caused a marked anaerobic metabolism as shown by the decrease in both muscle ATP and creatine phosphate and increase in muscle lactate. Free muscle glucose increased immediately postexercise and a further increase was noted 15 min later. There was a significant decrease (P<0.05) in proglycogen (57.1 +/- 22.2 mmol/kg dw) and macroglycogen (63.0 +/- 65.5 mmol/kg dw) during exercise. The proglycogen concentration tended to increase 15 min after exercise (19.9 +/- 27.3 mmol/kg dw; P = 0.06). The results from this study demonstrate that both proglycogen and macroglycogen contribute equally to glycogenolysis during intense exercise and suggest that glycogen resynthesis starts in the proglycogen pool.