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Hispin, a novel ribosome inactivating protein with antifungal activity from hairy melon seeds
1Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China. biochemistry@cuhk.edu.hk
Protein Expression and Purification
|October 31, 2002
Abstract:
A ribosome inactivating protein demonstrating a molecular mass of 21 kDa and a novel N-terminal sequence was isolated from seeds of the hairy melon. The purification procedure involved affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose and Mono S. The protein designated hispin inhibited translation in the cell-free rabbit reticulocyte lysate system with an IC50 of 165 pM and exhibited N-glycosidase activity. Antifungal activity was also observed.