Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiplexed, targeted gene expression profiling and genetic analysis on electronic microarrays.

Elaine M Weidenhammer1, Brenda F Kahl, Ling Wang

  • 1Department of Research and Development, Nanogen, Inc., 10398 Pacific Center Ct., San Diego, CA 92121, USA. eweidenhammer@nanogen.com

Clinical Chemistry
|October 31, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cultural Manifestations of Bereavement in a Chinese American Patient.

The primary care companion for CNS disorders·2026
Same author

A new deep field scan liquid chromatography-mass spectrometry method to identify host cell proteins in therapeutic proteins.

Journal of pharmaceutical and biomedical analysis·2025
Same author

Clinical and pathological features of pediatric peripheral T-cell lymphoma after solid organ transplantation.

Blood neoplasia·2025
Same author

Non-Tobacco Nicotine Use and Complications in Free Flap Repair in Head and Neck Cancer.

The Laryngoscope·2025
Same author

Insulinoma-Induced Mania.

The primary care companion for CNS disorders·2025
Same author

Efficacy of orexin antagonists for the management of major depressive disorder: A systematic review of randomized clinical trials.

Journal of affective disorders·2024

Electronic microarrays enable rapid, multiplexed gene expression profiling. This technology allows for specific target detection and quantification, offering advantages for genomic analysis.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioengineering

Background:

  • Electronic microarrays utilize microelectrode test sites for controlled movement and concentration of charged molecules like DNA and RNA.
  • A novel protocol for multiplexed gene expression profiling using electronic field-facilitated hybridization on these microarrays was developed.

Purpose of the Study:

  • To develop and validate a method for multiplexed gene expression profiling using electronic microarrays.
  • To demonstrate the capability of electronic microarrays for simultaneous multiple genetic analyses.

Main Methods:

  • Utilized T7 RNA polymerase-mediated amplification for target mRNA expression profiling from total cellular RNA.
  • Employed electronic field-facilitated hybridization on electronic microarrays for target detection.

Related Experiment Videos

  • Activated individual test sites to target specific subsets of probes and enable comparative sample analysis.

    • Main Results:

    • Achieved target detection within a 2-minute hybridization reaction.
    • Demonstrated the ability to detect twofold changes in target concentration across a wide dynamic range (approx. 64-fold).
    • Successfully performed simultaneous single-nucleotide polymorphism detection, gene expression profiling, and splicing isoform analysis on a single microarray.

    Conclusions:

    • Microelectronic array technology offers specific and quantitative target detection.
    • This technology presents advantages over existing methods for targeted gene expression profiling.
    • The platform is suitable for combinatorial genomics testing and complex molecular analyses.