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Updated: Aug 7, 2026

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
Published on: February 25, 2016
Cloning, functional expression, and mutational analysis of a cDNA for Lotus japonicus mitochondrial phosphate
Kazuki Nakamori1, Reona Takabatake, Yosuke Umehara
1Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan.
Abstract:
A full-length cDNA encoding mitochondrial phosphate transporter was isolated from symbiotic nodules of Lotus japonicus, a model legume. The gene appeared to be single-copied and its transcript was abundant in roots, symbiotic root nodules and shoots, in that order. The L. japonicus transporter has a conserved N-ethylmaleimide-reactive Cys residue. Site-directed mutagenesis and phosphate transport assaying in vitro showed that this Cys residue is preferable for the transport activity, although it can be replaced to some extent by Ser, but not by Ala.

