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Modification of dienes mutual inhibition test for epidemiological characterization of Pseudomonas aeruginosa isolates
Erik L Munson1, Michael A Pfaller, Gary V Doern
1Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
Abstract:
Pseudomonas aeruginosa is an important cause of community-associated and nosocomial infections related to exposure to aqueous environments. Such infections often occur in the setting of a common-source outbreak, in which case epidemiological characterization of isolates may be necessary. In this preliminary study, a modification of the Dienes mutual inhibition test, ordinarily used to assess the relatedness of swarming Proteus mirabilis strains, was used to study 15 P. aeruginosa isolates, with the results compared to those obtained by ribotype analysis. Complete concordance was noted between the results of the Dienes test and those of ribotyping. These observations suggest that further studies are warranted to assess the utility of the modified Dienes test as a simple, inexpensive, and reliable means for epidemiological typing of P. aeruginosa.
Insights
A modified Dienes test accurately identified relatedness between Pseudomonas aeruginosa strains, matching ribotyping results. This simple method shows promise for reliable bacterial epidemiological typing.
Area of Science:
- Microbiology
- Infectious Diseases
- Epidemiology
Background:
- Pseudomonas aeruginosa causes significant community and hospital-acquired infections, often linked to water sources.
- Outbreaks necessitate precise epidemiological characterization of bacterial isolates for source identification and control.
Purpose of the Study:
- To evaluate a modified Dienes mutual inhibition test for epidemiological typing of Pseudomonas aeruginosa.
- To compare the efficacy of the modified Dienes test with established ribotype analysis.
Main Methods:
- A modified Dienes mutual inhibition test was applied to 15 Pseudomonas aeruginosa isolates.
- Results were compared against those obtained using standard ribotype analysis.
Main Results:
- The modified Dienes test demonstrated complete concordance with ribotype analysis.
- This indicates high reliability in differentiating Pseudomonas aeruginosa strains.
Conclusions:
- The modified Dienes test is a potentially simple, cost-effective, and reliable method for the epidemiological typing of Pseudomonas aeruginosa.
- Further research is recommended to validate its broad applicability.
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