Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide

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Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
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Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Published on: August 19, 2014

M Radwanska1, S Magez, H Perry-O'Keefe

  • 1Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium. mradwanska@uctgsh1.uct.ac.za

Journal of Clinical Microbiology
|November 1, 2002
PubMed

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...