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An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
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Expanded-spectrum cephalosporin-resistant salmonella strains in Romania

Vivi Miriagou1, Roxana Filip, Gabriela Coman

  • 1Laboratory of Bacteriology, Hellenic Pasteur Institute. Department of Microbiology, Medical School, University of Athens, Athens, Greece. miriagou@mail.pasteur.gr

Journal of Clinical Microbiology
|November 1, 2002
PubMed

Insights

Fourteen Salmonella strains resistant to expanded-spectrum cephalosporins were found in Romanian infants. Most resistance stemmed from CMY-2 cephalosporinase, suggesting relatedness among these resistant Salmonella Typhimurium strains.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Expanded-spectrum cephalosporins are critical antibiotics for treating bacterial infections.
  • Emergence of resistance to these drugs poses a significant public health threat, especially in vulnerable populations like infants.
  • Salmonella species are common causes of gastroenteritis in children.

Purpose of the Study:

  • To investigate the prevalence and mechanisms of expanded-spectrum cephalosporin resistance in Salmonella isolates from infants with gastroenteritis.
  • To characterize the genetic relatedness of resistant Salmonella strains.

Main Methods:

  • Isolation and identification of Salmonella enterica serotypes Typhimurium and Heidelberg from infant stool samples.
  • Antimicrobial susceptibility testing, focusing on resistance to expanded-spectrum cephalosporins.
  • Detection of resistance genes, including CMY-2 cephalosporinase and SHV-5 beta-lactamase.
  • Plasmid analysis and pulsed-field gel electrophoresis (PFGE) for strain typing.

Main Results:

  • Fourteen resistant Salmonella isolates (13 S. Typhimurium, 1 S. Heidelberg) were identified between October 2000 and February 2001.
  • CMY-2 cephalosporinase, encoded by a nonconjugative plasmid, was the primary mechanism of resistance in 12 S. Typhimurium isolates.
  • One S. Typhimurium isolate produced an SHV-5 beta-lactamase, and the S. Heidelberg isolate's resistance mechanism was not detailed.
  • PFGE analysis indicated that the CMY-2-producing S. Typhimurium isolates were genetically related.

Conclusions:

  • The study identified a significant proportion of expanded-spectrum cephalosporin-resistant Salmonella in infants with gastroenteritis in Romania.
  • CMY-2 cephalosporinase is a key determinant of this resistance, often plasmid-mediated and associated with related S. Typhimurium strains.
  • These findings highlight the need for surveillance of antimicrobial resistance in pediatric populations and the potential for clonal spread.

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