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Real-time fluorescence-based PCR for detection of malaria parasites
May-Ann Lee1, Chiew-Hoon Tan, Lay-Tin Aw
1Defence Medical Research Institute, Defence Science and Technology Agency, National University of Singapore, Singapore 117597. nmiv13@nus.edu.sg
Journal of Clinical Microbiology
|November 1, 2002
Abstract:
A fluorescence-based real-time 5' nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.