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Ocular surface inflammation induced by Propionibacterium acnes
Tomo Suzuki1, Yoichiro Sano, Osamu Sasaki
1Department of Ophthalmology, Kyoto Prefectural University of Medicine Kajii-cho, Kawaramachi-Hirokoji Kamigyo-ku, Kyoto City 602-0841, Japan. tsuzuki@ophth.kpu-m.ac.jp
Cornea
|November 1, 2002
Summary
This study shows that bacteria can trigger a delayed-type hypersensitivity (DTH) response in the cornea, similar to skin reactions. This finding suggests a potential cause for ocular surface inflammation.
Area of Science:
- Ophthalmology
- Immunology
- Microbiology
Background:
- * The bacterium is commonly found in ocular tissues like the eyelid and conjunctiva.
- * It is known to cause granulomatous endophthalmitis and stimulate inflammation.
- * Previous studies show it induces delayed-type hypersensitivity (DTH) and granulomas in other organs.
Purpose of the Study:
- * To investigate if the bacterium can induce a DTH response specifically in the cornea.
- * To explore the bacterium's role in ocular surface inflammation.
Main Methods:
- * Lewis rats were immunized with heat-killed bacteria and challenged to assess DTH response.
- * Bacterial suspension was injected into the corneal stroma, with observations at 6, 24, and 48 hours.
- * Corneal tissues were examined histologically and immunohistochemically for cell infiltration (CD4, CD8).
Main Results:
- * Rats immunized with bacteria showed significant ear swelling, indicating a DTH response.
- * Corneal injection led to massive cellular infiltration and stromal edema within 48 hours.
- * Histology revealed a DTH-like pattern of neutrophil and mononuclear cell infiltration, with CD4 T-cells predominating.
Conclusions:
- * The bacterium can indeed induce a delayed-type hypersensitivity response in the cornea.
- * This bacterium may be a contributing factor to ocular surface inflammation.