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Published on: November 6, 2014
[Effect of interleukin-1beta on aromatase activity in human osteoblast-like cells]
Xiaoxin Ma1, Yan Li, Shulan Zhang
1Department of Obstetrics and Gynecology, Second College of China Medical University, Shenyang 110004, China.
Objective:
To study the stimulation of interleukin (IL)-1beta and interleukin-1 receptor antagonist (IL-1ra) on aromatase activity in human osteoblast cells.
Methods:
In the present study, the effect of IL-1beta and IL-1ra on aromatase (Arom) activity and mRNA expression in human osteoblast-like cells was investigated by [(3)H] water released upon the conversion of [1beta-(3)H] androstenedione to estrone and the reverse-transcription polymerase cain reaction (RT-PCR) method. The experimental group is divided into four group: G1 (IL-1beta 1 microgram/L), G2 (10 microgram/L), G3 (IL-1beta 10 microgram/L + IL-1ra 500 microgram/L) and G4 (IL-1ra 500 microgram,/L).
Results:
The activity of G1 and G2 resulted in (4 211 +/- 348) pmol/10(6) cell.h(-1) and (4 958 +/- 231) pmol/10(6) cell.h(-1), respectively. IL-1ra neutralized the increased arom activity to control level in G3 (3 652 +/- 203) pmol/10(6) cell.h(-1). Arom mRNA expression was increased with the dose increasing of IL-1beta.
Conclusion:
These findings suggest that IL-1beta stimulates arom activity through the IL-1 receptor and addition of IL-1beta increased arom activity in a dose-dependent manner.
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