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Expression of B7 costimulator in eight human malignant hematological cell lines and its significance
Liping Jia1, Xiaoyan Ke, Jing Wang
1The Third Hospital, Beijing University, Beijing 100083, China.
Objective:
To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.
Methods:
Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.
Result:
Eight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.
Conclusion:
Multiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.