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Cloning of full length cDNA sequence of the mouse ameloblastin
Shuping Gu1, Junnan Shi, Han Liu
1Department of Oral Medicine, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Objective:
Screening for special genes of matrix proteins of dentin and enamel of mouse dental germ.
Methods:
A cDNA library of dental germ of mouse was screened by differential display. The interesting clones were sequenced.
Results:
Six positive clones were isolated from the cDNA library. The sequence of one of the six positive clones was homologous with the ameloblastin sequence of rat. There are 497 homologous base pairs between the 526 base pairs sequenced by pTriplEX 3' primer of this clone and the 32-580 sequence of the rat ameloblastin gene; and there are 533 homologous base pairs between the 567 base pairs sequenced by pTriplEX 5' primer of this clone and the 1285-1854 sequence of the rat ameloblastin gene.
Conclusions:
The full length cDNA sequence of the mouse ameloblastin was cloned.