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Sulfite supported lipid peroxidation in propofol emulsions
Max T Baker1, Deborah J Dehring, Marc S Gregerson
1Department of Anesthesia, University of Iowa, Iowa City 52242, USA. max-baker@uiowa.edu
Anesthesiology
|November 2, 2002
Summary
Sodium metabisulfite in propofol emulsions promotes lipid peroxidation, increasing thiobarbituric acid reacting substances (TBARS). Propofol and ascorbic acid partially or fully inhibit these sulfite-induced reactions, respectively.
Area of Science:
- Pharmaceutical Chemistry
- Drug Formulation
- Oxidative Stability
Background:
- Sodium metabisulfite is an antimicrobial agent in propofol emulsions.
- Sulfite ions can cause autooxidation and lipid peroxidation.
- This study investigates sulfite's reactivity in propofol emulsions.
Purpose of the Study:
- To evaluate sulfite reactivity in propofol emulsions.
- To determine the impact of sulfite on lipid peroxidation.
- To assess the role of propofol and EDTA in mitigating sulfite-induced reactions.
Main Methods:
- Comparison of commercial EDTA and sulfite propofol emulsions.
- Analysis of thiobarbituric acid reacting substances (TBARS), sulfite, and pH in emulsions.
- Utilized modified thiobarbituric acid method, spectrophotometric sulfite analysis, and glass electrode pH measurement.
Main Results:
- TBARS were detected in sulfite propofol emulsions but not in EDTA emulsions.
- Incubation increased TBARS in sulfite emulsions, while EDTA emulsions remained stable.
- Metabisulfite significantly increased TBARS, with partial inhibition by propofol and complete inhibition by ascorbic acid.
- Metabisulfite addition decreased emulsion pH, with partial inhibition by propofol and complete inhibition by ascorbic acid.
Conclusions:
- Sulfite promotes lipid peroxidation in soybean oil-based propofol emulsions.
- Propofol exhibits partial inhibitory effects on sulfite-induced lipid peroxidation.
- Ascorbic acid effectively inhibits sulfite-induced lipid peroxidation and pH decline.