Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin-like growth factors decrease catecholamine content in PC12 rat pheochromocytoma cells.

L A Bach1, K S Leeding

  • 1University of Melbourne, Department of Medicine, Austin & Repatriation Medical Centre, Heidelberg, Victoria, Australia. l.bacj@unimelb.edu.au

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|November 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IGF-binding proteins.

Journal of molecular endocrinology·2017
Same author

Scaling of the mean and variance of population dynamics under fluctuating regimes.

Theory in biosciences = Theorie in den Biowissenschaften·2014
Same author

Diabetes is a major risk factor for mortality after lung transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
Same author

Stereotactic radiosurgery for treatment of Cushing disease: an Australian experience.

Internal medicine journal·2012
Same author

Advanced glycation end-products (AGEs) and functionality of reverse cholesterol transport in patients with type 2 diabetes and in mouse models.

Diabetologia·2012
Same author

Environmental fluctuations and level of density-compensation strongly affects the probability of fixation and fixation times.

Bulletin of mathematical biology·2010
Same journal

Determinants of Infant Participation and Non-Participation in Primary Prevention Trials.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2026
Same journal

Inflammatory Profile of Lipedema: A Comparative Evaluation with Obese and Normal-weight Women.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2026
Same journal

Comparative Effects of Experimental Diabetes Mellitus and Glucocorticoid-Induced Hypercortisolemia on Pancreatic and Adrenal Endocrine Architecture: A Pathological Study in Rats.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2026
Same journal

Psychiatric Symptoms in Women with Mild Autonomous Cortisol Secretion Versus Non-Functioning Adrenal Adenomas: A Cross-Sectional Study.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2026
Same journal

Antisemitism as a Chronic Cardiometabolic, Endocrine, and Glutamatergic Stressor Requiring Psychometric Monitoring.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2026
Same journal

Severe Acute Respiratory Syndrome Coronavirus-2 Infection and New Changes in Thyroid Function Parameters during Hospitalization and Follow-up.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2026
See all related articles

Insulin-like growth factors (IGFs) decrease catecholamine levels in PC12 cells via the IGF-I receptor. Dexamethasone increases catecholamine synthesis, but IGFs abolish the dexamethasone-induced rise in norepinephrine.

Area of Science:

  • Neuroscience
  • Endocrinology
  • Cell Biology

Background:

  • Insulin-like growth factors (IGFs) influence cell proliferation and differentiation.
  • IGFs modulate catecholamine release in adrenal medullary cells.
  • Dexamethasone enhances catecholamine synthesis in PC12 cells.

Purpose of the Study:

  • To investigate the effects of IGFs and dexamethasone on catecholamine content in PC12 cells.
  • To elucidate the role of the IGF-I receptor in mediating these effects.
  • To understand the interplay between IGFs and dexamethasone in regulating catecholamine synthesis.

Main Methods:

  • PC12 cells were incubated with IGF-I, IGF-II, [Leu27]IGF-II, and/or dexamethasone.
  • Catecholamine content (dopamine and norepinephrine) was measured.

Related Experiment Videos

  • Tyrosine hydroxylase mRNA, protein, and activity were assessed.

    • Main Results:

    • IGF-I and IGF-II significantly decreased dopamine and norepinephrine content.
    • [Leu27]IGF-II did not affect catecholamine levels, suggesting mediation via the IGF-I receptor.
    • Dexamethasone increased dopamine and norepinephrine levels; IGFs abolished the dexamethasone-induced increase in norepinephrine but not dopamine.

    Conclusions:

    • IGFs decrease catecholamine content in PC12 cells through the IGF-I receptor.
    • Dexamethasone upregulates catecholamine synthesis, with complex interactions with IGFs.
    • The regulation of catecholamine levels by IGFs involves intricate synthetic and/or degradative pathways.