Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new fluorescent quantitative polymerase chain reaction technique.

Wang Shengqi1, Wang Xiaohong, Chen Suhong

  • 1Beijing Institute of Radiation Medicine, Beijing 100850, China. sqwang@nic.bmi.ac.cn

Analytical Biochemistry
|November 5, 2002
PubMed
Summary

A novel complex probe utilizing fluorescence resonance energy transfer (FRET) enables sensitive, real-time gene detection. This method accurately quantifies genetic material over a wide dynamic range, ideal for diagnostics and research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DeepTriage-CN: integrating clinical text with vital signs for emergency department admission prediction in an aging population.

Scientific reports·2026
Same author

Real-time prediction of trauma-induced coagulopathy using an inverted transformer (trauma-former): a methodological feasibility and simulation study based on the ADEMP framework.

BMC medical research methodology·2026
Same author

Quantitative assessment and prognostic value of myocardial work indices in sepsis-induced cardiomyopathy patients with left ventricular systolic dysfunction.

Cardiovascular ultrasound·2026
Same author

Improving the Effectiveness of Adolescent Idiopathic Scoliosis (AIS) Screening: A Prospective Study.

Spine·2025
Same author

The ultrafine powder of atractylodis macrocephalae rhizoma improves immune function in naturally aging rats by regulating the PI3K/Akt/NF-κB signaling pathway.

Frontiers in pharmacology·2025
Same author

Corrigendum to "Sirtuin 1 alleviates microglia-induced inflammation by modulating the PGC-1α/Nrf2 pathway after traumatic brain injury in male rats" [Brain Res. Bull. 185 (2022) 28-38].

Brain research bulletin·2025

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Real-time gene detection is crucial for various biological applications.
  • Existing methods may lack sensitivity, specificity, or dynamic range.
  • Fluorescence Resonance Energy Transfer (FRET) offers a potential mechanism for sensitive detection.

Purpose of the Study:

  • To design and synthesize a novel complex probe for real-time gene detection.
  • To investigate the FRET properties and optimization of the complex probe.
  • To evaluate the sensitivity, specificity, and dynamic range of the developed probe.

Main Methods:

  • Design and synthesis of a complex probe comprising a fluorescent reporter probe and a quenching probe.
  • Utilizing FRET principles for signal generation upon template hybridization.

Related Experiment Videos

  • Optimization of reaction conditions including probe proportion and magnesium ion concentration.

    • Main Results:

    • The complex probe demonstrated efficient quenching in the absence of a template.
    • Fluorescence intensity was directly proportional to template quantity.
    • Optimal conditions identified: 1:1 probe ratio, 3mmol/L Mg2+.
    • High sensitivity (10^2 copies) and a broad dynamic range (10^2-10^9 copies) were achieved.

    Conclusions:

    • The novel complex probe provides a sensitive, accurate, and specific method for real-time gene quantification.
    • The probe is easily synthesized and exhibits thorough quenching.
    • This method is applicable for detecting virus infection levels, transgenic copy numbers, and single nucleotide polymorphisms.