Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interrelation between hydration and interheadgroup interaction in phospholipids.

Israel R Miller1, Diana Bach, Ellen J Wachtel

  • 1Department of Biological Chemistry, The Weizmann Institute of Science, P.O.B. 26, 76100 Rehovot, Israel. bmiller@weizmann.weizmann.ac.il

Bioelectrochemistry (Amsterdam, Netherlands)
|November 5, 2002
PubMed
Summary

Biological membranes

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The RNA-binding protein PRRC2B preserves 5' TOP mRNA during starvation to maintain ribosome biogenesis during nutrient recovery.

Nucleic acids research·2025
Same author

Local Environment of Sc and Y Dopant Ions in Aluminum Nitride Thin Films.

ACS applied electronic materials·2024
Same author

Loss-of-function cancer-linked mutations in the EIF4G2 non-canonical translation initiation factor.

Life science alliance·2023
Same author

Structural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins.

Chemical science·2023
Same author

Identifying a selective inhibitor of autophagy that targets ATG12-ATG3 protein-protein interaction.

Autophagy·2023
Same author

Cholesterol solubility in mixed DMPE/DMPC bilayers as determined by small angle X-ray scattering.

Biophysical chemistry·2023

Area of Science:

  • Membrane biophysics
  • Lipid bilayer hydration

Background:

  • Membrane stability and ionic conductivity are hydration-dependent.
  • Specific water molecules strongly bind to lipid headgroups, influencing membrane properties.

Purpose of the Study:

  • To investigate the role of hydration in lipid bilayer stability and function.
  • To quantify water molecule orientation and binding in phospholipids (PL).

Main Methods:

  • Differential scanning calorimetry (DSC) to detect unfreezable water.
  • Polarized Fourier-transform infrared spectroscopy (FTIR) to study water molecule orientation.
  • Molecular modeling to simulate membrane interactions.

Main Results:

  • Cholesterol increases unfreezable water in PL bilayers.

Related Experiment Videos

  • Seven to eight water molecules orient on phosphatidyl serine (PS) headgroups, with increased binding at phase boundaries.
  • Electrostatic interactions between charged layers containing PS can induce conformational changes and dehydration.

    • Conclusions:

    • Hydration significantly impacts lipid bilayer properties and biological membrane function.
    • Cholesterol and ion interactions modulate water binding and membrane behavior.
    • These findings offer insights into membrane fusion mechanisms.