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Identification of the ribosomal proteins present in the vicinity of globin mRNA in the 40S initiation complex

Yoshiaki Takahashi1, Takashi Mitsuma, Shigeru Hirayama

  • 1Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University, Asahimachi-doori Niigata 951-8518. yoshiaki@clg.niigata-u.ac.jp

Journal of Biochemistry
|November 6, 2002
PubMed

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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
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