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[Effects of cartilage-derived growth factor on cultured rabbit chondrocytes]
Jinfeng Ma1, Xiaoliang Chen, Yingzhen Wang
1Department of Orthopaedics, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Objective:
To investigate the effect of cartilage-derived growth factor (CDGF) on cultured rabbit chondrocytes, and the relation between CDGF, insulin-like growth factor-I (IGF-I), and proliferation and metabolism of chondrocytes.
Methods:
CDGF was extracted from chicken xiphoid in our laboratory. Chondrocytes were isolated from 3-week-old New Zealand male rabbits, and cultured in Ham's F-12 medium containing 10% fetal calf serum. The chondrocyts of second generation were treated with CDGF and/or IGF-I of different concentrations. With chloramine T method and MTT method, we compared the content of hydroxyproline in Ham's F-12 medium and observed proliferation and energy synthesis of chondrocytes.
Results:
CDGF and IGF-I both stimulated the proliferation and synthesis of hydroxyproline of chondrocytes dose-dependently. The optimal concentration of CDGF was 16 ng/ml and 32 ng/ml respectively, and that of IGF-I was 30 ng/ml. There was obvious synergic effect between CDGF and IGF-I.
Conclusion:
CDGF can stimulate the proliferation and collagen synthesis of chondrocytes and has synergistic effect with IGF-I.