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[Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901]
Xiaopeng Wu1, Zhanmin Wang, Bo Liu
1Department of General Surgery, Qilu Hospital, Shandong University, Ji'nan 250012, China.
Objective:
To establish a new extrahepatic growing hepatocellular carcinoma cell line.
Methods:
A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.
Results:
Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.
Conclusion:
EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.