Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel quality control slide for quantitative immunohistochemistry testing.

Seshi R Sompuram1, Vani Kodela, Keming Zhang

  • 1CytoLogix Corporation, Cambridge, Massachusetts 02139, USA. ssompuram@cytologix.com

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|November 6, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of a high-protein food for special medical purposes in midlife and older adults at risk of malnutrition: a multicenter, randomized, active-controlled clinical study.

The journal of nutrition, health & aging·2026
Same author

Multi-Level Variable Selection Using a BART-Enhanced Mixed-Effects Framework.

Statistics in medicine·2026
Same author

MOF gel network crosslinked mixed matrix membranes with engineered interface for high-efficiency Helium recovery.

Nature communications·2026
Same author

Abscess of the ligamentum teres hepatis and Arantius' ligament: A case report.

African health sciences·2026
Same author

Timing of Antifungal Treatment and Prognostic Factors in Talaromyces marneffei Infection: A Retrospective Analysis of HIV-Positive Cases.

Mycopathologia·2026
Same author

The Value of Internal Positive Controls and Synthetic Calibrators for Performance Assessment of Breast Carcinoma Estrogen Receptor Immunohistochemistry Assay.

Laboratory investigation; a journal of technical methods and pathology·2026

A new quality control method uses standardized peptide targets on slides to improve immunohistochemistry (IHC) staining consistency across labs. This innovation offers reproducible, unlimited standards for precise IHC analysis.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Histopathology

Background:

  • Immunohistochemistry (IHC) is crucial for diagnostics but suffers from variability.
  • Current IHC quality control methods lack standardization and reproducibility.
  • Standardizing IHC is essential for accurate patient diagnosis and treatment.

Purpose of the Study:

  • To introduce a novel quality control technology for improving IHC standardization.
  • To develop reproducible and unlimited antibody targets for IHC assays.
  • To assess the utility of peptide-based targets in detecting IHC staining variations.

Main Methods:

  • Developed standardized antibody targets using short constrained peptides attached to glass slides.
  • Integrated these peptide targets onto the same slides as patient samples for IHC staining.

Related Experiment Videos

  • Evaluated the peptides' ability to mimic native antigens and their stability during IHC procedures.

    • Main Results:

    • Peptide targets stained similarly to patient tissues, indicating they simulate native antigens.
    • The technology detected subtle changes in IHC staining efficacy.
    • Peptide targets demonstrated stability, resisting degradation from deparaffinization and antigen retrieval.

    Conclusions:

    • The novel peptide-based technology offers a reproducible method for IHC quality control.
    • This approach can improve intra- and interlaboratory standardization of IHC assays.
    • The technology holds potential for creating national standards to quantify IHC analytical variability.