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Related Experiment Videos

Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice.

Luanne L Peters1, Eleanor M Cheever, Heather R Ellis

  • 1The Jackson Laboratory, Bar Harbor, Maine 04609, USA.

Physiological Genomics
|November 7, 2002
PubMed
Summary

The Mouse Phenome Project provides essential hematologic and coagulation data for over 30 mouse strains. This high-throughput screening enables researchers to select optimal mouse models for their studies.

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Area of Science:

  • * Genomics and Phenomics
  • * Translational Medicine
  • * Animal Models

Background:

  • * The Mouse Phenome Project systematically collects phenotypic data from inbred mouse strains.
  • * Large-scale projects require efficient, high-throughput screening methods for comprehensive data acquisition.
  • * Standardized hematologic and coagulation testing protocols are crucial for mouse model research.

Purpose of the Study:

  • * To establish high-throughput hematologic and coagulation screening protocols for inbred mouse strains.
  • * To generate and disseminate comprehensive hematologic and coagulation data for over 30 mouse strains.
  • * To facilitate informed selection of mouse strains for research studies.

Main Methods:

  • * Complete blood counts were analyzed using the Advia 120 automated analyzer.

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  • * The Dade Behring BCS automated coagulation analyzer was adapted for mouse samples by reducing sample and reagent volumes.
  • * Seven automated coagulation assay procedures were developed and validated for mouse use.

    • Main Results:

    • * Hematologic and coagulation data were successfully generated for more than 30 inbred mouse strains.
    • * Adapted coagulation assays required minimal sample and reagent volumes, reducing costs and enabling multiple tests per animal.
    • * Significant variations in hematologic and coagulation parameters were observed across different inbred mouse strains.

    Conclusions:

    • * The developed high-throughput screening methods allow for efficient phenotyping of mouse hematologic and coagulation parameters.
    • * The generated data reveal substantial inter-strain variability, crucial for selecting appropriate mouse models.
    • * Freely available online data empower researchers to choose optimal mouse strains, advancing study design and research goals.