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Construction of BAC libraries derived from the ascidian Ciona intestinalis
Mari Kobayashi1, Masaru Matsuda, Shuichi Asakawa
1Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Japan.
Genes & Genetic Systems
|November 7, 2002
Abstract:
Large insert genomic bacterial artificial chromosome (BAC) libraries were constructed from a basal chordate, the ascidian Ciona intestinalis. Insert analyses of randomly selected clones indicated that in the first library the mean insert size was 135 kb and predicted a 15-fold coverage of the Ciona genome, and in the second library the mean insert size was 165 kb and predicted a 5-fold coverage of the genome. These first large insert genomic libraries of the ascidian should increase the speed of genomic analyses of basal chordates.