Observation of protein-derived (BSA) oxygen-centered radicals by EPR spin-trapping techniques
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Updated: Sep 28, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
Jean-Louis Clément1, Bruce C Gilbert, Antal Rockenbauer
1Laboratoire SREP UMR 6517, CNRS et Universit d'Aix-Marseille 1 et 3, France. jlclement@srepir1.univ-mrs.fr
Abstract:
Electron paramagnetic resonance (EPR) spin-trapping experiments, employing the novel spin-trap DEPMPO, provide evidence for the formation of protein-peroxyl radicals from the reaction of bovine serum albumin (BSA) or lysozyme with HO. in the presence of O2. Spin-trapping leads to the detection of anisotropic spectra of partially immobilized protein-peroxyl spin-adducts; positive identification is based on a novel spectrum simulation approach (through which broadened anisotropic spectra are simulated and compared with experiment) and by comparison of results with those obtained when MeO2. is trapped and the adduct frozen in a solid matrix.
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