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Related Experiment Videos

Dosage considerations in patch testing with liquid allergens.

Daniel W Shaw1, Hongbo Zhai, Howard I Maibach

  • 1University of California, San Diego, Department of Medicine, San Diego, CA 92103-8420, USA. dwshaw@ucsd.edu

Contact Dermatitis
|November 9, 2002
PubMed
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Dropper bottle choice impacts patch test accuracy. Inconsistent drop volumes and varying liquid properties affect allergen delivery, highlighting the need for careful selection based on reproducibility and chamber type.

Area of Science:

  • Dermatology
  • Allergy Testing
  • Formulation Science

Background:

  • Accurate allergen delivery is crucial for reliable patch testing.
  • Variability in dropper bottle performance can affect test results.
  • Understanding liquid properties and filter paper saturation is essential.

Purpose of the Study:

  • To assess the reproducibility of drop volumes from plastic squeeze dropper bottles.
  • To compare drop volumes across different liquid patch test solutions.
  • To determine filter paper saturation volumes for water and ethanol in common patch test chambers.

Main Methods:

  • Quantified drop volumes from various plastic squeeze dropper bottles.
  • Measured drop volumes of water, ethanol, and specific allergens.

Related Experiment Videos

  • Determined saturation volumes of filter papers (Finn and IQ Chambers) using water and ethanol.

    • Main Results:

    • Two recommended patch test dropper bottles showed poor reproducibility.
    • Aqueous allergens exhibited drop volumes similar to water; ethanol-based solutions produced smaller drops.
    • Water saturation volumes were 16-19 µL (Finn Chambers) and 29-35 µL (IQ Chambers); ethanol volumes were slightly lower.

    Conclusions:

    • Dropper bottle selection for patch testing requires consideration of drop volume reproducibility.
    • Allergen type influences drop volume, necessitating adjustments for different solutions.
    • Current recommended application volumes (15 µL for Finn, 25 µL for IQ Chambers) are appropriate as they are below saturation points.