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Published on: October 14, 2011
Transduction of concatemeric plasmids containing the cos site of Lactococcus lactis bacteriophage sk1
P Scott Chandry1, Sean C Moore, Barrie E Davidson
1Food Science Australia, Werribee, Victoria, Australia. scott.chandry@foodscience.afisc.csiro.au
Abstract:
Lactococcus lactis bacteriophage sk1 can transduce plasmids containing the phage cos site and surrounding DNA sequences at frequencies as high as 2x10(-3) transductants per PFU. Deletion analysis demonstrated that the presence of phage DNA spanning cos and putative R sites were the most important for efficient plasmid transduction. Inserts of 440 bp containing cos and the R sites were sufficient to induce transduction frequencies of 10(-4) transductants per PFU. The role of the R1 site was investigated by altering 14 of the 19 bases in the site. This resulted in a two-fold decrease in transduction frequency compared to a 26-fold decrease in transduction following deletion of the entire site. It was demonstrated that transducing plasmids were packaged as linear trimeric concatemers commencing at the cos site.
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