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p53, mdm2, and p21 expression in oral squamous cell carcinomas: relationship with clinicopathologic factors
Souichi Yanamoto1, Goro Kawasaki, Izumi Yoshitomi
1Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. syana@net.nagasaki-u.ac.jp
Objective:
The purpose of this study was to clarify the correlation of expression of cell cycle-associated gene proteins with clinicopathologic factors in oral squamous cell carcinoma (SCC).
Study Design:
Formalin-fixed paraffin-embedded tissues from 69 oral SCC cases and 10 normal mucosa cases were stained by immunohistochemistry (IHC) for p53, mdm 2, and p21 proteins.
Results:
We found p53, mdm 2, and p21 expression in 44 of 69 (63.8%), 25 of 69 (36.2%), and 37 of 69 (53.6%) oral SCCs, respectively. Ki-67-labeling index of combined p53(+)/mdm 2(+) expression cases was significantly higher than those that lacked combined expression (P =.004). Combined p53(+)/p21(+) expression showed a significant association with lymph node metastasis (P =.019). In survival analysis, combined p53(+)/p21(+) and p53(+)/mdm 2(+)/p21(+) expression was associated with poor clinical outcome (P =.018 and.012, respectively).
Conclusion:
Combined p53/mdm 2 expression was associated with tumor proliferation in oral SCC. Combined p53/p21 and p53/mdm 2/p21 expression may be a predictive factor in lymph node metastasis.