Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Imaging ocular immune responses by intravital microscopy.

James T Rosenbaum1, Stephen R Planck, Tammy M Martin

  • 1Casey Eye Institute, Department of Medicine-Division of Allergy, Immunology and Rheumatic Diseases, Department of Cell & Developmental Biology, Oregon Health and Science Center, Portland, Oregon, USA.

International Reviews of Immunology
|November 12, 2002
PubMed
Summary

The eye allows real-time observation of immune cell interactions, including leukocyte trafficking and adhesion, using advanced microscopy. These insights into ocular immune responses are crucial for understanding eye diseases and evaluating treatments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exogenous Nano-Silicon Treatment Improved the Low-Temperature Tolerance of Rice Seedlings.

Plants (Basel, Switzerland)·2026
Same author

Rediscovering eosinophil identity through tissue adaptation.

Nature immunology·2026
Same author

Long-term metabolic-adiposity burden and cardiovascular risk: the cumTyG-WHtR in Chinese adults.

BMC cardiovascular disorders·2026
Same author

Macular fibrosis secondary to neovascular age-related macular degeneration: from clinic to biology.

Progress in retinal and eye research·2026
Same author

MSC-EVs attenuate subretinal fibrosis in choroidal neovascularization through miR-21-5p-mediated inhibition of EMT and MMT and suppression of inflammation.

Journal of neuroinflammation·2026
Same author

BLIMP1 shapes germinal center B cell clonal diversity by gating chromatin accessibility during light-to-dark zone transition.

Nature immunology·2026

Area of Science:

  • Ophthalmology
  • Immunology
  • Microscopy

Background:

  • The eye provides a unique in vivo environment for studying cellular interactions.
  • Immune responses within the eye involve complex events like cell trafficking, adhesion, and antigen presentation.

Purpose of the Study:

  • To investigate immune cell behavior and interactions within the eye using intravital microscopy.
  • To analyze leukocyte-endothelial interactions in ocular microvascular beds.
  • To determine the timing of T cell trafficking and identify antigen-presenting cells in ocular tissues.

Main Methods:

  • Intravital microscopy of the conjunctiva, cornea, and iris.
  • Scanning laser ophthalmoscopy in rat and mouse models.
  • Real-time imaging of fluorescently labeled antigens and cells in the anterior chamber.

Related Experiment Videos

  • Analysis of leukocyte-endothelial interactions in retinal and choroidal circulations.

    • Main Results:

    • Detailed observation of leukocyte rolling and adhesion in unmanipulated ocular microvasculature.
    • Quantification of shear stress effects on leukocyte-endothelial adhesion.
    • Insights into T cell trafficking dynamics and potential antigen-presenting cells within the eye.

    Conclusions:

    • Intravital microscopy offers powerful tools for studying ocular immune responses in real time.
    • These methods provide valuable data on leukocyte behavior and antigen presentation in the eye.
    • The techniques are being adapted for human eye studies to understand tissue damage and treatment efficacy.