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Related Experiment Videos

Cell interactions with polyelectrolyte multilayer films.

L Richert1, Ph Lavalle, D Vautier

  • 1INSERM Unité 424, UFR d'Odontologie, Université Louis Pasteur, 11 rue Humann, F-67085 Strasbourg Cedex, France.

Biomacromolecules
|November 12, 2002
PubMed
Summary

Cell interactions with polyelectrolyte films depend on the outermost layer and serum presence. Poly(L-glutamic acid) (PGA) films prevent protein adsorption and cell adhesion, while poly(L-lysine) (PLL) films show increased adhesion.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Chemistry

Background:

  • Polyelectrolyte multilayer (PEM) films are versatile for surface modification.
  • Understanding cell-material interactions is crucial for biomaterial development.
  • Chondrosarcoma cells provide a model for studying cellular responses to novel surfaces.

Purpose of the Study:

  • To investigate the short-term interactions of chondrosarcoma cells with PEM films.
  • To determine the influence of the film's terminal layer (poly(L-lysine) (PLL) or poly(L-glutamic acid) (PGA)) on cell adhesion.
  • To assess the role of serum proteins in mediating cell-PEM film interactions.

Main Methods:

  • Fabrication of PEM films via alternate adsorption of PLL and PGA.
  • Characterization of films and protein adsorption using optical waveguide lightmode spectroscopy, quartz crystal microbalance, and zeta potential.

Related Experiment Videos

  • Measurement of cell detachment forces using the micropipet technique in serum-containing and serum-free media.

    • Main Results:

    • Serum presence significantly reduced chondrosarcoma cell detachment forces on PGA-ending films compared to PLL-ending films.
    • Adhesion forces on PLL-ending films decreased with increasing film thickness.
    • In serum-free conditions, PLL-ending films exhibited 40-100% higher adhesion forces, while PGA-ending films showed no cellular adherence.
    • PGA-ending films inhibited serum protein adsorption, whereas PLL-ending films showed significant protein adsorption.

    Conclusions:

    • Cell interactions with PEM films are tunable by the terminal layer chemistry.
    • The presence of serum proteins modulates cell adhesion to PEM films.
    • PEM film thickness and protein adsorption influence cell adhesion, offering strategies for controlling cell-material interfaces.