Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dissection of c-MOS degron.

Jun Sheng1, Akiko Kumagai, William G Dunphy

  • 1Division of Biology, 147-75, California Institute of Technology, 1200 East California Blvd, Pasadena, CA 91125, USA.

The EMBO Journal
|November 12, 2002
PubMed
Summary

The N-terminal Pro residue of c-MOS is not essential for its degradation in oocytes. Degradation depends on Ser-2 phosphorylation, not Pro, challenging previous models of c-MOS regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Regulation of Endogenous Transcription Factor Hubs at Single-Molecule Resolution.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Myosin-bound glycyrrhizic acid and liquiritin from Glycyrrhiza uralensis Fisch. attenuate thermal polycyclic aromatic hydrocarbon formation: spectroscopic and computational mechanistic insights.

Food chemistry·2026
Same author

Antagonistic modulation of myosin conformational dynamics by sanshool and smoke-derived aldehydes: A molecular basis for heterocyclic amine control in thermal processing.

Food chemistry·2026
Same author

Phytochemical Diversity, Mechanistic Pharmacology, and Therapeutic Potential of <i>Alpinia oxyphylla</i>.

Foods (Basel, Switzerland)·2026
Same author

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The <i>Cl1978</i> gene regulates delphinidin-based anthocyanin accumulation in a rare purple Wampee (<i>Clausena lansium</i>).

Food chemistry. Molecular sciences·2026

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • c-MOS (cytoplasmic-only MOS) is a MAP kinase kinase kinase crucial for oocyte maturation.
  • Protein concentration is regulated by conditional degradation.
  • The N-terminal proline (Pro) of c-MOS was previously thought to target it for degradation via the 'second-codon rule'.

Purpose of the Study:

  • To investigate the degradation signal (degron) of c-MOS in Xenopus oocytes.
  • To determine the role of the N-terminal Pro residue in c-MOS degradation.
  • To elucidate the mechanism regulating c-MOS protein stability.

Main Methods:

  • Analysis of c-MOS degradation signals in Xenopus oocytes.
  • Site-directed mutagenesis to alter the N-terminal Pro residue and Ser-2 phosphorylation status.
  • Assessment of c-MOS protein stability and phosphorylation levels.

Main Results:

  • The N-terminal Pro residue of c-MOS is dispensable for its degradation.
  • Replacing Ser-2 with Gly renders the N-terminal Pro irrelevant for degradation.
  • N-terminal Pro downregulates Ser-2 phosphorylation, which normally halts c-MOS degradation.

Conclusions:

  • The N-terminal Pro residue of c-MOS does not function as a degron recognized by ubiquitin ligases.
  • c-MOS degradation is primarily regulated by the phosphorylation status of Ser-2.
  • This finding refutes the 'second-codon rule' model for c-MOS degradation and highlights a novel regulatory mechanism involving proline and phosphorylation.

Related Experiment Videos