Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue factor deficiency causes cardiac fibrosis and left ventricular dysfunction.

R Pawlinski1, A Fernandes, B Kehrle

  • 1Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 12, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-sectional survey of resistant priority pathogen carriage in the hospital setting in Timor-Leste 2020-2021.

Antimicrobial resistance and infection control·2026
Same author

Energy Spectrum of Ultrahigh-Energy Cosmic Rays across Declinations -90° to +44.8° as Measured at the Pierre Auger Observatory.

Physical review letters·2026
Same author

Search for the Anomalous Events Detected by ANITA Using the Pierre Auger Observatory.

Physical review letters·2025
Same author

Inference of the Mass Composition of Cosmic Rays with Energies from 10^{18.5} to 10^{20}  eV Using the Pierre Auger Observatory and Deep Learning.

Physical review letters·2025
Same author

From fixing to connecting: parents' experiences supporting adult children with eating disorders.

Journal of eating disorders·2024
Same author

TRGT-denovo: accurate detection of <i>de novo</i> tandem repeat mutations.

bioRxiv : the preprint server for biology·2024

Low levels of tissue factor (TF) in mice led to fatal hemorrhages and heart defects, suggesting TF is crucial for cardiac hemostasis and preventing heart damage.

Area of Science:

  • Cardiovascular Biology
  • Hemostasis and Thrombosis
  • Molecular Medicine

Background:

  • Tissue factor (TF) initiates coagulation via the extrinsic pathway (TF:FVIIa).
  • The intrinsic pathway involves Factor IXa (FIXa).
  • TF's role in cardiac hemostasis is not fully understood.

Purpose of the Study:

  • To investigate the role of tissue factor (TF) in cardiac hemostasis.
  • To determine the impact of reduced TF levels on cardiac function and lifespan.
  • To explore the potential of TF as a secondary hemostatic barrier in the heart.

Main Methods:

  • Generated mice with low levels of human TF (1% of wild-type) on an mTF(-/-) background.
  • Assessed lifespan, incidence of hemorrhage, and cardiac pathology in low-TF mice.

Related Experiment Videos

  • Measured left ventricular function using intracardiac methods.
  • Compared cardiac findings in low-TF, low-FVII, and FIX(-/-) mice.

    • Main Results:

    • Low-TF mice exhibited significantly shorter lifespans due to spontaneous fatal hemorrhages.
    • All low-TF mice developed cardiac defects, including hemosiderin deposition and fibrosis.
    • Left ventricular function was reduced by 30% in low-TF mice.
    • Similar cardiac pathology was observed in mice with low Factor VII (FVII) levels.
    • FIX(-/-) mice (hemophilia B model) had normal hearts, implicating TF and FVII, not FIX, in this cardiac phenotype.

    Conclusions:

    • Reduced TF levels impair cardiac hemostasis, leading to hemorrhage and fibrosis.
    • TF expression by cardiac myocytes may serve as a critical secondary hemostatic barrier.
    • Impaired hemostasis due to low TF or FVII causes cardiac damage, distinct from hemophilia B.