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Platelet-derived growth factor-D expression in developing and mature human kidneys.
Siribha Changsirikulchai1, Kelly L Hudkins, Tracy A Goodpaster
1Department of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Kidney International
|November 13, 2002
Summary
Platelet-derived growth factor D (PDGF-D) is newly identified in human kidney tissues, appearing in developing nephrons and adult visceral epithelial cells. This finding suggests PDGF-D plays a role in kidney development and may be involved in vascular pathology.
Area of Science:
- Nephrology
- Vascular Biology
- Cell Biology
Background:
- Platelet-derived growth factor (PDGF) family members regulate biological processes.
- PDGF-D is a recently identified PDGF family member.
- PDGF-D expression was previously noted in vascular wall cells, but not in human kidneys.
Purpose of the Study:
- To investigate the localization of PDGF-D in human kidney tissues.
- To determine the role of PDGF-D in kidney development and pathology.
Main Methods:
- Immunohistochemistry was used to examine PDGF-D expression in fetal and adult human kidney tissues.
- Western blotting and RT-PCR confirmed PDGF-D protein and gene expression.
- Antibody specificity was verified through peptide absorption.
Main Results:
- PDGF-D was detected in developing nephron epithelial cells and differentiating glomerular visceral epithelial cells.
- In adult kidneys, PDGF-D was found in visceral epithelial cells, arterial smooth muscle cells, and injured tubules.
- PDGF-D protein and gene expression were confirmed by Western blotting and RT-PCR.
Conclusions:
- This study provides the first localization of PDGF-D in human kidneys.
- PDGF-D expression suggests a role in kidney development.
- Potential paracrine interactions involving PDGF-D and kidney cells were proposed.