Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coating sections for electron microscopic autoradiography: a stripping technique using liquid emulsion.

R W Burry, R S Lasher

    Journal of Microscopy
    |August 1, 1975
    PubMed
    Summary

    A new electron microscope autoradiography coating method improves emulsion distribution on grids. This technique offers moderate resolution with minimal loss in image quality for biological samples.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Maintenance of rat taste buds in primary culture.

    Chemical senses·2001
    Same author

    p21(ras) stimulates pathways in addition to ERK, p38, and Akt to induce elongation of neurites in PC12 cells.

    Journal of neuroscience research·2001
    Same author

    Corticotropin releasing factor induces proliferation of cerebellar astrocytes.

    Journal of neuroscience research·2000
    Same author

    Gangliosides and growth factor receptor regulation.

    Biochemical Society transactions·2000
    Same author

    Sodium channel Na(v)1.6 is localized at nodes of ranvier, dendrites, and synapses.

    Proceedings of the National Academy of Sciences of the United States of America·2000
    Same author

    Specificity controls for immunocytochemical methods.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2000

    Area of Science:

    • Microscopy
    • Cell Biology
    • Radiochemistry

    Background:

    • Electron microscope autoradiography is crucial for visualizing cellular processes.
    • Traditional coating methods can present challenges in emulsion distribution and resolution.
    • Optimizing techniques is essential for accurate biological sample analysis.

    Purpose of the Study:

    • To introduce a novel coating technique for electron microscope autoradiography.
    • To evaluate the resolution and image quality of the new method.
    • To provide a practical approach for enhanced autoradiographic analysis.

    Main Methods:

    • A dipping method for forming an emulsion film was combined with grid-based section coating.
    • A formvar support film was used to separate the biological section from the emulsion crystals.

    Related Experiment Videos

  • Ilford L-4 emulsion and Microdol-X development were employed with light gold sections.

    • Main Results:

    • The formvar layer ensured a random distribution of emulsion crystals.
    • The technique achieved a resolution of 150 nm, determined by the half-distance method.
    • A slight reduction in image quality was observed with biological samples.

    Conclusions:

    • This technique offers a practical solution for moderate-resolution electron microscope autoradiography.
    • It combines the benefits of dipping methods with the ease of grid coating.
    • Minimal loss in resolution and image quality makes it suitable for various applications.