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Functional characterization of the human spermidine/spermine N(1)-acetyltransferase gene promoter
Hideyuki Tomitori1, Mitsuru Nenoi, Kazuei Mita
1Department of Clinical Biochemistry, Graduate School of Pharmaceutical Sciences, Chiba University, 1-33, Yayoi-cho, Inage-ku, Chiba, Japan.
Biochimica Et Biophysica Acta
|November 13, 2002
Abstract:
Spermidine/spermine N(1)-acetyltransferase (SSAT), the key enzyme of polyamine catabolism, is induced by antiproliferative stresses. We analyzed the 5' flanking region of the human SSAT gene, and clarified that the binding of Sp1 to the GC-box located 42 to 51 bp upstream from the transcription start site is essential for transcription in HeLa S3 cells. A polyamine-responsive element (PRE) seemed to be responsible for the elevated transcription after X-ray irradiation.