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Related Experiment Videos

How much LH do the Leydig cells see?

B P Setchell1, P Pakarinen, I Huhtaniemi

  • 1Pediatric Endocrinology Unit, Karolinska Hospital, Stockholm, Sweden. brian.setchell@adelaide.edu.au

The Journal of Endocrinology
|November 14, 2002
PubMed
Summary
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Luteinizing hormone (LH) concentrations reaching rat Leydig cells are only 10% of blood levels, suggesting endothelial cells restrict access. This implies Leydig cells are highly sensitive to LH or testicular endothelial cells modulate its action.

Area of Science:

  • Reproductive Endocrinology
  • Cell Biology
  • Physiology

Background:

  • Leydig cells are crucial for testosterone production.
  • Luteinizing hormone (LH) regulates Leydig cell function.
  • Understanding LH bioavailability in the testis is key to steroidogenesis regulation.

Purpose of the Study:

  • To quantify LH concentrations experienced by Leydig cells in vivo.
  • To investigate the impact of gonadotrophin-releasing hormone (GnRH) on testicular LH levels.
  • To correlate LH levels with testosterone production in rat testes.

Main Methods:

  • Measurement of LH in testicular interstitial fluid, seminiferous tubular fluid, and blood plasma (peripheral and testicular venous).
  • Intravenous injection of GnRH or saline in rats.

Related Experiment Videos

  • Comparison of hormone concentrations before and after stimulation.
  • Quantification of testosterone levels in testicular cells, interstitial fluid, and venous plasma.

    • Main Results:

    • LH concentrations in interstitial fluid were ~10% of plasma levels.
    • GnRH injection caused a smaller LH rise in interstitial fluid compared to plasma.
    • Testosterone levels significantly increased in cells and interstitial fluid post-GnRH.
    • No significant change in LH or testosterone was observed in tubular fluid.

    Conclusions:

    • Testicular endothelial cells likely restrict LH entry into the interstitial fluid.
    • Leydig cells may possess high sensitivity to LH or their function is modulated by testicular endothelial cells.
    • This differential LH availability impacts steroidogenesis regulation within the testis.