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Detection of a novel pattern of connexin 43 immunoreactivity responsive to dehydration in rat hypothalamic
1Unidad de Neurobiologia Aplicada (CEMIC-CONICET), Av. Galván 4102, 1431, Buenos Aires, Argentina.
Abstract:
Immunocytochemical expression of Connexin 43 (Cx 43) in the rat Supraoptic Nucleus was analyzed following dehydration, using sequence-specific anti-Cx 43 antibodies (designated 13-8300, 71-0700, and sc-9059) that exhibit differential recognition of Cx 43. Punctate and longitudinally arranged immunostaining patterns of Cx 43 labeling, as evidenced by antibody sc-9059, was detected overlaying the nucleus of magnocellular neuroendocrine cells. This novel form of longitudinally arranged Cx 43 immunoreactivity was modified by dehydration and halothane exposure, but not lactation.
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