Related Experiment Video
Updated: Aug 20, 2026

RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
Published on: September 27, 2016
Quercetin, apoptosis, heat shock
Joanna Jakubowicz-Gil1, Jolanta Rzymowska, Antoni Gawron
1Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, ul. Akademicka 19, 20-033, Lublin, Poland. jjgil@biotop.umcs.lublin.pl
Abstract:
The present study was designed to investigate the correlation between the expression level of Hsp27 and Hsp72 and induction of apoptosis in HeLa cells in response to quercetin treatment. Treatment of HeLa cells with quercetin or with 1hr period of hyperthermia (42 degrees) increased the number of apoptotic cells. Inhibition of the expression of Hsp72 and Hsp27 in tumour cells by anti-sense oligonucleotides, enhanced the induction of apoptosis by quercetin. Heat shock itself had little effect on apoptotic cell death in these cells, but when combined with quercetin treatment, caused a significant increase in the number of apoptotic cells. These results suggest that the reduction of Hsps expression in the HeLa cell line promotes the induction of apoptosis by quercetin.
Related Concept Videos
Regulation of the Unfolded Protein Response
Caspases
The Extrinsic Apoptotic Pathway
The Intrinsic Apoptotic Pathway
Cellular Injury V: Apoptosis and Autophagy

