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Purification and crystallization of coconut globulin cocosin from Cocos nucifera
D Balasundaresan1, R Sugadev, M N Ponnuswamy
1Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600025, India.
Biochimica Et Biophysica Acta
|November 14, 2002
Abstract:
Cocosin is a legume class reserve protein found in coconut endosperm. Using coconut endosperm, two methods of purification were done. Crystallization was achieved by vapor diffusion (hanging drop) method using MPD, PEG 3350 and PEG 4000 as precipitants. X-ray diffraction data to 3.5-A resolution were collected using Mar345 image plate detector system. Crystals of cocosin grown under 20% MPD, are rhombohedral with space group R3 and cell dimensions a=92.829 A, b=92.829 A, c=215.290 A.