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Related Concept Videos

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jun 25, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

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Published on: April 14, 2010

Multiple control of interleukin-8 gene expression.

Elke Hoffmann1, Oliver Dittrich-Breiholz, Helmut Holtmann

  • 1Institute of Pharmacology, Medical School Hannover, Germany.

Journal of Leukocyte Biology
|November 14, 2002
PubMed
Summary
This summary is machine-generated.

Interleukin-8 (IL-8) expression varies greatly, increasing significantly during inflammation. Its production is regulated by gene promoter activity, transcription factors, and mRNA stabilization, controlling leukocyte attraction to injury sites.

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Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Interleukin-8 (IL-8) is a key human chemokine, foundational to the chemokine superfamily.
  • IL-8 exhibits highly variable expression, being low in healthy tissues but significantly upregulated during inflammation.

Purpose of the Study:

  • To elucidate the regulatory mechanisms governing Interleukin-8 (IL-8) transcription and mRNA stabilization.
  • To understand how external stimuli modulate IL-8 expression.

Main Methods:

  • Analysis of signaling pathways controlling IL-8 gene transcription.
  • Investigation of mRNA stabilization mechanisms.
  • Examination of the roles of nuclear factor-kappaB, JUN-N-terminal protein kinase, and p38 mitogen-activated protein kinase pathways.

Main Results:

  • Maximal IL-8 production results from a combination of gene promoter derepression, transcriptional activation via NF-κB and JNK pathways, and mRNA stabilization by the p38 MAPK pathway.
  • These mechanisms allow for rapid and fine-tuned control of IL-8 secretion.

Conclusions:

  • The coordinated regulation of IL-8 transcription and mRNA stability is crucial for controlling leukocyte recruitment.
  • Understanding these pathways provides insights into inflammatory responses and leukocyte trafficking.