Related Experiment Video

Updated: Sep 4, 2026

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

Linking DNA damage to cell cycle checkpoints

Temesgen Samuel1, H Oliver Weber, Jens Oliver Funk

  • 1Laboratory of Molecular Tumor Biology, University of Erlangen-Nuremberg 91052 Erlangen, Germany.

Cell Cycle (Georgetown, Tex.)
|November 14, 2002
PubMed

Insights

Cell cycle checkpoints are crucial for monitoring DNA damage and replication, ensuring proper cell cycle progression. Understanding these mechanisms is key to comprehending cancer development.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cell cycle checkpoints are essential signal transduction pathways.
  • These pathways monitor DNA integrity and replication status.
  • Dysregulation of checkpoints is implicated in various diseases, including cancer.

Purpose of the Study:

  • To elucidate the functions of core components in the cell cycle response to DNA damage.
  • To provide a framework for understanding these critical cellular processes.
  • To explore the relevance of DNA damage response pathways to cancer origins.

Main Methods:

  • This study employs a theoretical framework approach.
  • It synthesizes existing knowledge on cell cycle regulation and DNA damage.
  • The focus is on the functional roles of key molecular players.

Main Results:

  • A comprehensive framework for understanding cell cycle checkpoint functions is presented.
  • The core components involved in DNA damage response are identified and their roles clarified.
  • The link between checkpoint function and cancer etiology is highlighted.

Conclusions:

  • Cell cycle checkpoints are vital for maintaining genomic stability.
  • Defects in DNA damage response pathways contribute to oncogenesis.
  • Further research into these mechanisms could reveal novel cancer therapeutic targets.

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle08:30

Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

Published on: December 22, 2023

Related Concept Videos

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...