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A neoantigenic determinant in the D-dimer fragment of fibrin

E V Lugovskoy1, I N Kolesnikova, P G Gritsenko

  • 1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha street, Kiev 01601, Ukraine.

Thrombosis Research
|November 15, 2002
PubMed

Monoclonal antibody (mAb) III-3b binds D-dimer with K(d)=1.4 x 10(-10) M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bbeta134-190, presumably in Bbeta155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III-3b may be used as a tool for immunodiagnostic quantification of D-dimer in blood plasma.

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