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Updated: Sep 28, 2026
A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
Published on: June 5, 2014
[Detection of Mycobacterium tuberculosis in lung cancer tissue by indirect in situ nested PCR]
Lan-Ying Song1, Wen-Sheng Yan, Tong Zhao
1Department of Pathology, First Military Medical University, Guangzhou 510515, China.
Objective:
To localize Mycobacterium tuberculosis DNA (TB-DNA) in lung cancer tissue and to investigate the possible relationship of this bacterial infection with the development of lung cancer.
Methods:
A sensitive and specific indirect in situ nested PCR (ISNPCR) was used to identify and localize TB-DNA in 15 formalin fixed paraffin-embedded lung cancer tissue specimens, which had been demonstrated to be positive for TB-DNA by conventional PCR.
Results:
Positive granules of TB-DNA in brown color was found mainly in the cytoplasm of the alveolar epithelial cells, pulmonary macrophages, inflammatory cells and a few tumor cells within lung cancer tissues.
Conclusion:
Mycobacterium tuberculosis infection may play a role in the pathogenesis of lung cancer.
