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[Effects of recombinant sea anemone cytotoxin on vascular smooth muscle cell proliferation in rats]
Ping Ouyang1, Xiao-Yu Jiang, Wen-Li Yang
1Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou, 510515, China.
Objective:
To observe the effects of recombined sea anemone cytotoxin (rSAC) on vascular smooth muscle cell proliferation in rats rSAC.
Methods:
Aortic smooth muscle cells (ASMCs) of rats were cultured in vitro and stimulated with rSAC of different concentrations, and were observed in comparison with the control group. The ratio of cell proliferation was determined with non-radioactive MTS/PES assay.
Results:
The ratio of cell proliferation rate was 0.802+/-0.055 in the control group, and was 0.115+/-0.014, 0.213+/-0.016, 0.335+/-0.097, 0.663+/-0.060, 0.678+/-0.129, and 0.738+/-0.072 in rSAC groups corresponding to rSAC concentrations of 100, 10, 1 mg/ml and 100, 10, 1 ng/ml respectively. It was shown that rSAC significantly inhibited the cell proliferation when the concentration used was above 100 ng/mg (P<0.05), in a dose-dependent manner as compared with the control group (P<0.05).
Conclusion:
rSAC can significantly inhibit the vascular smooth muscle cell proliferation in rats.
