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[Construction and identification of cDNA library of E.coli mRNA with poly(A) tracts]
Zi-You Hu1, Wen-Li Ma, Qing-Hua Wu
1Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA
|November 16, 2002
Abstract
Objective:
To construct and identify the cDNA library of E.coli mRNA with poly(A) tracts.
Methods:
The cDNA library of E.coli was constructed by restriction display-PCR (RD-PCR) technique, followed by sequencing and bioinformatics analyses.
Results:
cDNA library of E.coli mRNA with poly(A) tracts was successfully constructed, and 66 gene fragments were sequenced.
Conclusion:
The constructed cDNA library of E.coli mRNA with poly(A) tracts contains a low rate of repetition and is of high quality.