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A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
Published on: April 14, 2010
[Preliminary study of sodium butyrate-induced expression of lung resistance-related protein in K562 cells]
Ning Li1, Xin-Hua Qian, Ying-Min Yao
1Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To investigate the effect of sodium butyrate (NaB) on the expression level of lung resistance-related protein (LRP) in K562 cells.
Methods:
K562 cells, used as an in vitro model system, were treated with NaB to induce differentiation of the cells. LRP mRNA expression and protein levels in the cells were detected by reverse transcriptase-PCR (RT-PCR) and flow cytometry, respectively.
Results:
A 1-day treatment with 2 mmol/L NaB induced the gene expression of LRP to increase to the maximum in the cells, and a 3-day treatment caused the corresponding protein level to increase to the maximum.
Conclusion:
Both the mRNA level and the protein expression of LRP can be induced by NaB in K562 cells.
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