Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Voltage-activated proton currents in human lymphocytes.

Tom Schilling1, Alexander Gratopp, Thomas E DeCoursey

  • 1Institute of Physiology, Humboldt University, Tucholskystrasse 2, D 10117 Berlin, Germany.

The Journal of Physiology
|November 16, 2002
PubMed
Summary

Voltage-activated proton currents were identified in human T and B lymphocytes, showing distinct properties and modulation by pH and inhibitors. These proton channels are crucial for cellular functions like the respiratory burst.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Respiratory syncytial virus as an independent driver of a subsequent influenza epidemic in Berlin before and after the COVID-19 pandemic.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Near-Fatal Asthma Due to Severe Airway Mucus Plugging in a 12-Year-Old Boy.

Klinische Padiatrie·2026
Same author

TGFβ links EBV to multisystem inflammatory syndrome in children.

Nature·2025
Same author

Biophysical Properties of Somatic Cancer Mutations in the S4 Transmembrane Segment of the Human Voltage-Gated Proton Channel hH<sub>V</sub>1.

Biomolecules·2025
Same author

Proton reactions: From basic science to biomedical applications.

Biophysical journal·2024
Same author

Management of Busulfan-Induced Lung Injury in Pediatric Patients with High-Risk Neuroblastoma.

Journal of clinical medicine·2024

Area of Science:

  • Immunology
  • Cell Physiology
  • Biophysics

Background:

  • Proton (H+) currents play vital roles in cellular processes.
  • Voltage-gated ion channels are critical for cell signaling.
  • Lymphocytes (T and B cells) are key immune cells with complex electrophysiological properties.

Purpose of the Study:

  • To investigate voltage-activated proton currents in human peripheral blood T and B lymphocytes and Jurkat E6-1 cells.
  • To characterize the properties and regulation of these proton currents.
  • To explore the functional implications of proton currents in lymphocytes.

Main Methods:

  • Tight-seal voltage-clamp recordings were employed.
  • Proton currents (H+) were studied in T lymphocytes, B lymphocytes, and Jurkat E6-1 cells.

Related Experiment Videos

  • Effects of pH gradients, intracellular calcium, and heavy metals (Zn2+, Cd2+) were assessed.

    • Main Results:

    • Voltage-activated H+ currents were observed in all studied human lymphocyte types.
    • Proton currents exhibited high selectivity for protons and were modulated by extracellular and intracellular pH.
    • Inhibitors like Zn2+ and Cd2+ affected current kinetics and voltage dependence.
    • Phorbol ester (PMA) stimulation differentially modulated H+ currents in T and B lymphocytes.

    Conclusions:

    • The identified voltage-activated proton channels in lymphocytes possess unique properties suitable for charge compensation during cellular responses.
    • These findings reveal a novel electrophysiological mechanism in lymphocytes with potential implications for immune cell function and disease.
    • Further research into proton channel function could elucidate their role in the respiratory burst and other lymphocyte activities.