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Updated: Sep 28, 2026
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the
Mariagrazia Perilli1, Bernardetta Segatore, Maria Rosaria De Massis
1Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, Italy.
Abstract:
A bla(TEM-92) gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli. Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.
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