The origin of DNA replication and Fieller's problem
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Updated: Sep 28, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
Published on: October 27, 2011
Young K Truong1, Rona S Scott, Jean-Michel H Vos
1Department of Biostatistics, CB#7400, University of North Carolina, Chapel Hill, NC 27599-7400, USA. truong@bios.unc.edu
Abstract:
The paper considers the statistical problem of estimating the origin of DNA replication within the human ribosomal DNA (rDNA) locus and the issue of assessing the standard error of the estimate. Based on mapping the cumulative replication index (CRI), two different modelling schemes are suggested and investigated. The statistical problem of constructing a confidence interval for the origin of DNA replication is related to Fieller's problem of obtaining a confidence interval for the ratio of two normal means. Standard normal theory, the delta and bootstrap methods are used to estimate the standard error of the estimate of the origin of DNA replication, as well as the variation of the replication rate.
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