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Identification of Staphylococcus xylosus isolated from C57BL/6J-Nos2(tm1Lau) mice with dermatitis
Young Suk Won1, Hyo Jung Kwon, Goo Taeg Oh
1Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, Korea.
Abstract:
Coagulase negative staphylococci (CNS) are significant pathogens, particularly in medical device related infections and in immunocompromised patients. Five CNS strains were isolated from 5 NOS2 knockout mice with dermatitis. Histologically, granulomatous dermatitis was found in the skin around the ear with epidermal ulceration. Dermal lesions included pustules, necrosis, and accumulations of neutrophils and macrophages. Isolates of the bacterial strains were identified to be Staphylococcus xylosus by the API STAPH kit and 16S-23S intergenic transcribed spacer-PCR. These results demonstrate the potential of this organism to be an opportunistic pathogen in immunocompromised mice.
Insights
Coagulase-negative staphylococci (CNS) can cause infections, especially in immunocompromised individuals. This study identifies Staphylococcus xylosus as a potential opportunistic pathogen in mice with dermatitis.
Area of Science:
- Microbiology
- Immunology
- Veterinary Pathology
Background:
- Coagulase-negative staphylococci (CNS) are recognized as significant pathogens, frequently implicated in medical device-associated infections and infections in immunocompromised patients.
- Dermatitis is a common condition affecting various animal models, potentially leading to secondary bacterial infections.
Purpose of the Study:
- To identify the bacterial species responsible for dermatitis in NOS2 knockout mice.
- To evaluate the pathogenic potential of isolated CNS strains in an immunocompromised host model.
Main Methods:
- Isolation and bacterial culture from skin lesions of affected mice.
- Histopathological examination of skin biopsies to characterize dermatitis.
- Bacterial identification using API STAPH system and 16S-23S intergenic transcribed spacer-PCR.
Main Results:
- Five CNS strains were isolated from five NOS2 knockout mice exhibiting dermatitis.
- Histopathology revealed granulomatous dermatitis with epidermal ulceration, pustules, necrosis, and inflammatory cell infiltration.
- Bacterial isolates were identified as Staphylococcus xylosus.
Conclusions:
- Staphylococcus xylosus was identified as the causative agent of dermatitis in this cohort of immunocompromised mice.
- These findings highlight the opportunistic pathogenic potential of Staphylococcus xylosus, particularly in immunocompromised hosts.
- The study provides insights into bacterial coinfections in genetically modified mouse models with inflammatory conditions.